Innovative Methodology For Multiple Gene Insertion Into Plants - Easier Plant Improvement

Agricultural Research Service (ARS) scientists in Albany, California, have found a way to streamline the process that scientists use to insert multiple genes into a crop plant, developing a reliable method that will make it easier to breed a variety of crops with vastly improved traits.

The technology is expected to speed up the process for developing new varieties of potatoes, rice, citrus and other crops that are better equipped to tolerate heat and drought, produce higher yields and resist a myriad of diseases and pests. Crops with greater resistance to pathogens and insects could greatly reduce pesticide use and prevent billions of dollars in crop losses.

“Making genetic improvements that were difficult or impossible before will be much easier because we can now insert not just one or two genes, but multiple genes, into a plant in a way that will lead to predictable outcomes,” said Roger Thilmony, an ARS molecular biologist in Albany.

A paper describing the achievement by Thilmony, James Thomson, an ARS geneticist in Albany, and Ray Collier, a former ARS postdoctoral researcher, was published recently in the August issue of The Plant Journal.

The GAANTRY gene stacking technology will be freely available to anyone interested, and a commercial firm in the US is planning to use it to introduce multiple genes into potatoes to make them more resistant to late blight, which is caused by a fungus-like organism. Late blight can destroy entire fields and force some farmers to spray fungicides up to 15 times a year.

“We have struggled to put multiple late blight resistance genes into potatoes for years. They are very long, complex genes, and with existing technologies it’s been extremely difficult. But the GAANTRY technology will help us tremendously,” said Craig Richael, a director of research and development for J.R. Simplot Co., an Idaho-based company that produces French fries, frozen vegetables, fertilizer, turf grass seed and other products.

Scientists over the years have modified the genetics of soybeans, corn, canola and other crop plants to develop varieties that tolerate specific herbicides and resist insect pests. But those traits were controlled by one or two genes, and in most crop plants, important traits such as cold and drought tolerance, yield and seed production are almost always controlled by multiple genes. Inserting more than two or three genes into the same site on a plant chromosome has been notoriously difficult.

The researchers’ unique platform stabilizes large “stacks” of DNA needed for conferring key traits, allowing researchers to insert suites of genes “so precisely that no unintended DNA is added or lost during the process,” says Thomson.

“Before this, assembling 10 genes to insert into a new line would be difficult or impossible, but this technology basically stabilizes the stack and makes for results that are more stable and much easier to predict,” Thilmony said.

Read the report in The Plant Journal.

This technology offers some very smart options for plant improvement, and is potentially likely to be assessed similarly as the CRSPR system whereby derived plant lines are not assessed as GM plants, easing regulatory approvals. 

The Agricultural Research Service is the U.S. Department of Agriculture's chief scientific in-house research agency. Daily, ARS focuses on solutions to agricultural problems affecting America. 

Each dollar invested in agricultural research through the ARS results in $20 of economic impact.

That is a very good return on the investment.   I wonder if those returns are achieved in Australian institutions?

[ modified from publicly available press release of ARS ]

Cannot Stop the Advance of GM Technology

Agricultural Biotechnology Council of Australia (ABCA) chair Ken Matthews says some Australian governments are “gutless” when it comes to giving farmers access to genetically modified (GM) crops.

The former secretary of both the Australian agriculture and transport departments made the no-nonsense observation in a detailed outline of global and domestic attitudes on biotechnology at the National Farmers' Federation Congress in Canberra last week.  

Mr Matthews summed up by saying Australia suffers from not having a more objective, science-based discussion about agricultural biotechnology.  “It’s really important that Australia has practicing farmers speak up for agricultural biotechnology because it’s practicing farmers that will be persuasive.”  The ongoing anti-GM campaign is one of the “big risks” facing the technology’s development, he said.

“There is a great suspicion of science and scientists in public debate in Australia and there has been a very effective campaign by NGOs (non-government organisations) which has influenced public opinion.

“As a result, what worries me is that environmentally responsible farmers - who tend in many other areas to be leaders of farm opinion - can often be ambivalent about GM.  “The pro-GM constituency among farmers is therefore not as strong as it could be in Australia.”

Governments need to lead

Mr Matthews said attitudes held by the general public, consumers, environmentalists and media were also central problems in the GM debate.

But his strongest criticism was reserved for various governments that refuse to allow GM crops to be cultivated, despite overwhelming scientific evidence.

“Some governments in Australia are – and I use this word carefully – gutless,” he said.

“There are total bans on GM in South Australia, Tasmania and the ACT,” he said.

“There are moratoriums in WA and NSW but there are certainly exemptions for that. In my view they (total bans) aren’t rationally based; they aren’t properly founded in science.

“Those moratoria need to be ended and we need to avoid further mandatory labelling requirements, unless they can be justified.”

Hinting at the recent high profile legal case involving neighbouring organic and GM farmers, Steve Marsh and Mike Baxter in Kojonup WA, Mr Matthews said the problem with reconciling organic standards and GM crops also “needs to be tackled”.

He said there were three inescapable truths about biotechnology: the first being that it provides a critical opportunity to lift the productivity, profitability and sustainability of Australian agriculture over the next 20 years.

Secondly, he said biotechnology was critical to meeting rapidly rising global food needs. 

And thirdly, biotechnology will inevitably continue to attract “suspicion and opposition” in some community sectors, slowing its development.

Mr Matthews said that opposition was based on four key concerns: food safety; environmental damage; “agrochemical industry domination” and “a roundup of ethical and religious issues”.

However, he said science delivered a very clear message about the safety of GM technology.

GM safety scientifically proven

Mr Matthews said more than 100 of the world’s independent science oversight bodies, including “very authoritative and credible organisations” in the US and Europe, shared a consensus that GM crops are “as safe as conventional varieties - and often safer because of the extensive approval process they need to go through”.  “The scientific evidence is overwhelmingly in favour of GM safety, assuming that we continue with the sound regulatory processes that we have,” he said.  “There have now been 3 trillion meals (consumed) involving or including GM and there’s been not one recorded case of harm.  “There have been 70 billion animals which have been feeding on GM feed of some sort, and not one recorded case of harm.”

Mr Matthews said a recent European review of over 1700 scientific studies around the world about environmental impacts of GM concluded that there was little or no evidence GM crops caused negative environmental impacts.

“To me, as a student of public policy, it’s really interesting that the environment movement is very keen for science to be heard in the climate change debate - but where the science is very clear, no one seems to want to know about biotechnology,” he said.

Mr Matthews faced a question from the floor at the NFF Congress from NSW Farmers executive councillor Gai Marshall, referring to the controversial Seralini rat study on GM corn, which purported to find the GM feed caused tumour growth. Ms Marshall said she wasn’t against growing GM but wanted to see greater traceability.

Mr Matthews said the Seralini piece of science had been retracted. “It has been rejected, it has been withdrawn,” he said.  “The peer review, the second time around, found it totally discredited.”

A solution to global food security?

Mr Matthews said the problems GM could solve were “unprecedented in history”.

He said there are huge farm business opportunities from potential GM products like self-fertilising plants that could “revolutionise agriculture and certainly would decouple agriculture from the oil industry”.

GM crops also had a key role to play in aiding the future food demand task as the world’s population grows from 7.2 billion now to 9.6 billion by 2050, with decreasing land and water to develop globally - 800 million people already go to bed hungry each night and 1 billion people are chronically undernourished, he said.

“In the meantime, if I can be a bit provocative, there are some pretty comfortable western based NGOs which continue to oppose and to slow down biotechnology, on non-scientific, without evidence grounds,” he said.

Mr Matthews said a striking example of that argument was the fact 250 million children are currently suffering from vitamin A deficiency but GM food crops with existing solutions “are having trouble getting mobilised”.  “Norman Borlaug, said to be the father of the green revolution, said once that if the naysayers do stop agricultural GM they might actually precipitate those famines and crises they’ve been predicting for years,” he said.

Mr Matthews said the technology’s uptake was unprecedented, with 79 per cent of global soybean area and 70pc of global cotton area now GM.  “Growers are certainly not changing their mind and going back after they trial GM,” he said.

“Over the last 200 years there have been several waves of innovation in agriculture such as mechanisation, conventional plant breeding, chemical fertilisers and chemical herbicides.

“But none of them has been adopted as rapidly as GM seeds and I can tell you that animal-based GM is coming up very fast behind them.  “One eighth of global farm land is now GM, so this is a sort of mega bus that will not be stoppable.”


Mr Matthews said GM offers varieties with production benefits like faster growth rates and yields, drought tolerance, pesticide reduction and nutrient efficiency.

He said exciting work is also happening to generate varieties with consumer and health benefits like foods with fewer saturated fats, zero allergens, increased dietary fibre, reduced natural toxins, higher protein levels, built in vaccines, cholesterol management, and vitamin A.

“Benefits do flow to big biotech companies but perversely the regulatory costs - as a consequence of pressure from the opponents of GM - now make approvals just out of reach for anyone but the big firms,” he said.  “As an example, a single new GM trait may now cost $139 million, including $35 million just for the approvals part of that, and around the world it takes five-and-a-half years on average to gain a single new approval.

“Are those costs and time problems squeezing out public good innovations such as environmental public health innovations or small market GM innovations?”

Need to build trust

Mr Matthews said Australia grew GM canola and GM cotton and had great strengths in the area, with a world class regulatory system and plant breeding expertise.

But he said a three-part plan was needed to help overcome the slow progress of biotechnology development.

He said a constituency of biotechnology supporters was needed to build understanding of the potential benefits to farmers, consumers, the environment and society as a whole.  “We need to build community confidence and trust in Australia’s regulatory arrangements,” he said.  “We do need to be respectful of ethical concerns about biotechnology, but at the same time we need to give voice to the beneficiaries, and I think of those kids in Africa.

“When people are talking grandly about ethical concerns about biotechnology I worry about starving kids in Africa.  “We need to focus research more on benefits to consumers, to the environment, to society and we need to find some champions.” 

[ based on an article in the online edition of Qld Country Life 4 Nov 2014]